Kobra Karimi; Mohammad taghi Beigi nassiri; Mahmood Nazari; khalil Mirzadeh
Volume 21, Issue 4 , January 2020, , Pages 431-439
Abstract
The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific ...
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The present study was conducted to evaluate the effect of Tribulus Tresstrise (TT) herb on sex ratio of semen in Arabic Khouzestan ram using real time-qPCR technique using 18 rams in a completely randomized design with 3 treatments. The SRY and PLP genes were amplified to isolate the specific fragments of Y- and X- chromosome sequences. The treatments included: i) the control group (0% TT), ii) Diet containing 15 g/kg TT, iii) Diet containing 30 g/kg TT. Sperm sampling was taken from all rams at 10 month of age and blood sampling was performed at 8 and 10 month of age. The results showed that expression rate of SRY gene increased with increasing TT level and rams that received 30 g/kg TT diet had the highest SRY gene expression and PLP gene expression decreased with increasing TT level (p-value =0.004). There was positive correlation between Testosterone concentration and SRY gene expression at 8 (0.65) and 10 (0.59) month of age, and the relationship between PLP gene expression and Testosterone concentration was negative and -0.61 and -0.66 at 8 and 10 month of age, respectively (p-value= 0.006). The results indicated that adding Tribulus Tresstrise herb to the ram diet increases the SRY gene expression and also sperm containing Y chromosome. In other words, it increases the sex ratio toward male gens in Arabic Khozestan ram by increasing the androgen hormones.
tahereh mohammadabadi; Khalil Mirzadeh
Volume 19, Issue 4 , March 2018, , Pages 765-776
Abstract
This research was conducted to investigate the effect of processing sesame straw with low steam pressure, sulfuric acid and enzyme mixture on digestibility and ruminal fermentation, protozoa population, rumination and some blood parameters of Arabi sheep. Twelve sheep were fed with control diet (without ...
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This research was conducted to investigate the effect of processing sesame straw with low steam pressure, sulfuric acid and enzyme mixture on digestibility and ruminal fermentation, protozoa population, rumination and some blood parameters of Arabi sheep. Twelve sheep were fed with control diet (without sesame straw), diet containing sesame straw processed with 2.4 % sulfuric acid and low steam pressure at 130 °C for 120 minutes and 3 gr/kg DM enzyme, diet containing sesame straw processed by sulfuric acid and enzyme and diet containing sesame straw processed with water. The dry matter and organic matter intake, daily gain, feed conversion, apparent digestibility of dry matter, organic matter, NDF and ADF did not differ between treatments.The processing did not affect ammonia nitrogen, pH, and rumen protozoa species of sheep. Chewing, rumination and resting times were different between treatments and the greatest rumination time (491.67 minutes per day) was for processing with acid and enzyme (P<0.05). The eating, rumination and chewing time for dry matter, ADF and NDF, and blood glucose and urea of sheep were not different. Therefore, according to results of this experiment, sesame straw processed with water, processed with sulfuric acid and low steam pressure and enzymes and processed with acid and enzyme can be used at the expense of wheat straw in the sheep nutrition.
parviz ordouny; khalil mirzadeh; tahereh mohammadabadi; mohammad bojarpoor
Volume 19, Issue 3 , November 2017, , Pages 601-612
Abstract
To evaluate the effect of different levels of wild pistachio leaves on liver enzymes, bloodparameters and performance indicators of broiler chickens, 192 one-day old Ross 308 broiler chickens in the completely randomized design, including 4 treatments, 4 replicates and 12 chicks (both sexes), per ...
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To evaluate the effect of different levels of wild pistachio leaves on liver enzymes, bloodparameters and performance indicators of broiler chickens, 192 one-day old Ross 308 broiler chickens in the completely randomized design, including 4 treatments, 4 replicates and 12 chicks (both sexes), per experimentalunits were used. The experimental treatments includedrationscontaining levelsofzero (control), 1, 2, and 3 percent of wild pistachio leaf powder. The results of this experiment showed that effect of wild pistachio leaf on feed intake and daily gain significantly reduced compared to control treatment in the initial period (P˂0.05), but feed intake and daily gain were not significant in the growth period and total period.The feedconversion ratio was not significantly affected (P > 0.05). The effect of experimental treatments was not significant on AST and ALT, but the ALP enzyme was significantly reduced by the experimental treatments (P˂0.05). The amount of glucose, total cholesterol and HDL cholesterol of serum were not significantly affected. The effect of experimental treatments on triglyceride and LDL cholesterol serum reduction was significant (P˂0.05). According to the results, wild pistachio leaf can be used as an additive plant with reducing feature on liver enzymes and triglyceride and LDL cholesterol in poultry diets.
Behrouz Yarahmadi; Morteza Chaji; Mohammad Boujarpour; Khalil Mirzadeh; Morteza Rezaei
Volume 18, Issue 3 , October 2016, , Pages 447-459
Abstract
This experiment was carried out the aim of the effect of sainfoin forage and forage:concentrate ratios on feed intake, nitrogen balance,ruminal biohydrogenation and milk fatty acid profile in dairy ewes using of eight Lori breed ewes according to a replicated 4 × 4 Latin square design. Treatments ...
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This experiment was carried out the aim of the effect of sainfoin forage and forage:concentrate ratios on feed intake, nitrogen balance,ruminal biohydrogenation and milk fatty acid profile in dairy ewes using of eight Lori breed ewes according to a replicated 4 × 4 Latin square design. Treatments were adjusted from one to four according to forage:concentrate ratios of 35:65 with and without sainfoin and 65:35 with and without sainfoin. Dry matter intake was not significant among experimental diets. Retention of N was higher in ewes that receiveddiets consist of forage:concentrate ratios of 35:65 with sainfoin and 65:35 with and without sainfoin (P<0.05). The high concentrate diet with sainfoinwas the most vaccenic acid concentration accompanied by the less concentrations of stearic acid in ruminal liquor (P<0.05). Apparently biohydrogenation ofhigh concentrate diet with sainfoin (treatment one) decreased for oleic, linoleic and linolenic acid with 10.2, 12.3and 7.1%, respectively (P<0.05). Experimental diets effect were significant on vaccenic acid, conjugated linoleic acid, linoleic acid and linolenic acidconcentrationsin milk fat (P<0.05).In base onobtained the results,feeding of dairy ewes with high concentrate diets consist of sainfoin forage were an important due to increased conjugated linoleic acid and vaccenic acid concentration in milk fat.
Masoume Manteghi; Morteza Mamouei; Saleh Tabatabaei Vakili; Jamal Fayazi; Khalil Mirzadeh
Volume 18, Issue 2 , June 2016, , Pages 367-375
Abstract
The Effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°C was evaluated. Semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body ...
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The Effect of various amount of soybean lecithin replaced with egg yolk to protect the ram semen in times of one hour after semen collection, as well as 24 and 48 hours after the semen store at 5°C was evaluated. Semen was collected weekly for 8 weeks from 10 rams with 2-3 years old and average body weights of 64 kg. Semen samples were pooled and divided into 4 parts. Each part was diluted with one of the tris base extender containing 0.5, 1 and 1.5 percent of soybean lecithin and 14 percent of egg yolk. The motility, viability, morphological abnormalities and membrane integrity of spermatozoa and pH of semen were evaluated. The results showed that 1.5 percent of soybean lecithin improved the motility and viability of spermatozoa at the first time (P< 0.05). At the 48 hours after the semen store in 1 and 1.5 percent of soybean lecithin the sperm viability improved when compared with egg yolk treatment (P<0.05). Overall, soybean lecithin is the appropriate replacement with egg yolk and improved the semen quality in times of study. With regard to limitations of egg yolk using with animal source in ram extender, it was recommended to be using the soybean lecithin as the plant source.
Elahe Rezaei; Saleh Tabatabaei Vakili; Khalil Mirzadeh; Somayeh Sallary; Mehdi Zarei
Volume 18, Issue 1 , April 2016, , Pages 151-160
Abstract
This study was carried out to evaluate the effect of different levels of anise seed on the performance, immune system, antioxidant activity and blood estrogen level of broiler chicks. A total of 192 day -old Ross 308 broilers were trained for 42 days in a completely randomized design with four treatments, ...
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This study was carried out to evaluate the effect of different levels of anise seed on the performance, immune system, antioxidant activity and blood estrogen level of broiler chicks. A total of 192 day -old Ross 308 broilers were trained for 42 days in a completely randomized design with four treatments, four replicates and 12 birds per each replicate. Treatments were included the levels of zero (control(, 0.3, 0.6 and 0.9 percent of anise seed in diet. SRBC was injected at day 28 and blood samples were collected from chickens at days 35 and 42 of rearing. Dietary anise supplementation had no positive effect on feed intake and weight gain of broilers. In starter period, the chicks fed by 0.3 percent of anise seed, had the least feed conversion ratio (P<0.05). However, anise seed levels had no positive effects on feed conversion ratio in grower and total rearing period. Anise seed caused the higher antibody titer against SRBC in day 35 of rearing (P<0.05). The cellular immunity did not improve by anise seed levels in broilers. The 0.3 percent of anise seed, caused the higher total antioxidant capacity of blood plasma than control chicks (P<0.05). The blood serum estrogen level was reduced in 0.3 and 0.6 percent, but increased in 0.9 percent of anise seed (P<0.05). Generally, however the anise seed dose not improves performance traits and cellular immunity of broilers, but increse the primary humeral immunity and total antioxidant capacity of chicken.
Parvin Saeedi; Saleh Tabatabaei; Somayeh Sallary; Khalil Mirzadeh; Mehdi Zarei
Volume 16, Issue 2 , October 2015, , Pages 157-166
Abstract
In order to evaluation the effect of Nigella Sativa supplementation on performance, immune response and some of blood parameters, a total of 192 one day old Ross 308 broilers were used in a completely randomize design with four treatments, four replicates and 12 observations in each replicate. Treatments ...
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In order to evaluation the effect of Nigella Sativa supplementation on performance, immune response and some of blood parameters, a total of 192 one day old Ross 308 broilers were used in a completely randomize design with four treatments, four replicates and 12 observations in each replicate. Treatments included the increasing levels of zero (control), 0.4, 0.8 and 1.2 percent Nigella sativa. In grower and whole period of experiment, the weight of birds received 0.4 and 0.8 percent of Nigella sativa was higher than control group birds (P<0.05). The supplementation of Nigella sativa in diet has not effect on feed intake and feed conversion ratio in starter, grower and total rearing periods. The weights of bursa and thymus in birds received 0.4 and 0.8 percent of Nigella sativa werehigher than control group birds (P<0.05). The weight of liver in birds belong to 0.4 percent of Nigella sativawas higher than control group (P<0.05). Addition of Nigella sativa in dietreduced the glucose, triglyceride, cholesterol, LDL and increased the HDL in blood serum. The use of Nigella sativa in dietary of birds caused the higher total white blood cells and lower heterophil: lymphocyte ratio. The birds which received 0.4 and 0.8 percent of Nigella sativa had the higher total antioxidant capacity than control group in blood plasma. In conclusion, the use of Nigella sativa up to 0.8 percent in diet, in addition to improvement the performance, increased the immune system activity and total antioxidant capacity in Ross 308 broiler chickens.